Furthermore, Nakahara (fimA type I\V) was not significantly different between the favorable and unfavorable outcome groups

Furthermore, Nakahara (fimA type I\V) was not significantly different between the favorable and unfavorable outcome groups. with Tween (PBST), then serum samples in PBST were added to the wells. After incubation at 4C overnight, the wells were washed with PBST, then filled with alkaline phosphatase\conjugated goat anti\human IgG (gamma\chain specific; Abcam, Cambridge, MA, USA) in PBST. After another incubation at 37C for 2?h, the wells were again washed with PBST, then an aliquot of p\nitrophenylphosphate at 1 mg/m; (Wako Pure Chemical Industries Ltd., Osaka, Japan) in 10% diethanolamine buffer was added to each well as a substrate and incubation was performed at 37C for 30?min. Optical density at 405?nm was measured using a microplate reader (iMark; Bio\Rad Laboratories Inc., Hercules, CA, USA). Values ?1 were considered to represent more than 2 standard deviations of the mean of the controls and defined as antibody\positive. Absolute serum antibody measurements were used to categorize the Estradiol dipropionate (17-Beta-Estradiol-3,17-Dipropionate) samples as positive or negative [8,9]. Statistical analysis Statistical analysis was performed using the spss software package, Estradiol dipropionate (17-Beta-Estradiol-3,17-Dipropionate) version 24.0 (SPSS Inc., Chicago, IL, USA). Values are expressed as the mean??standard deviation or median (minimum, maximum) for continuous variables, and as frequencies and percentages for discrete variables. Univariate analysis was performed to evaluate differences between the groups regarding baseline characteristics, risk factors, levels of serum CRP and serum IgG antibody titers to periodontal pathogens. Comparisons between the groups were made using an unpaired (%)?Never306 (573)Past100 (187)Current124 (232)Alcohol status, (%)?No274 (513)Occasionally109 (204)Daily128 (240)Hypertension, (%)401 (751)Diabetes mellitus, (%)129 (242)Dyslipidemia, (%)227 (425)Atrial fibrillation, (%)93 (174)Ischemic heart disease, (%)43 (81)Peripheral arterial disease, (%)18 (34)Congestive heart failure, (%)21 (39)CRP, mg/l, median (IQR)011 (004C036) Open in a separate window NIHSS?=?National Institutes of Health Stroke Scale; IQR?=?interquartile range; CRP?=?C\reactive protein; BMI?=?body mass index. Table 2 Comparison of clinical characteristics between favorable and unfavorable groups (%)??039Never192 (57)114 (579)?Past69 (205)31 (157)?Current75 (223)49 (249)?Alcohol status, (%)??012No175 (519)99 (503)?Occasionally65 (193)44 (223)?Daily92 (273)36 (183)?Hypertension, (%)246 (730)155 (787)016Diabetes mellitus, (%)79 (234)50 (254)063Dyslipidemia, (%)40 (188)53 (269)Estradiol dipropionate (17-Beta-Estradiol-3,17-Dipropionate) AUNY67 (serotype c), ATCC25586 (subspecies ATCC10953 (subspecies ATCC33238 were more frequently detected in the unfavorable compared with the favorable group, while detection of antibodies to the other examined periodontal pathogens was not significantly different between the groups. Table 3 Detection of serum IgG antibody titers to periodontal pathogens in favorable and unfavorable groups after stroke (%)ATCC33277 (fimA type I)163 (484)95 (482)096 HW24D1 (fimA type II)141 (418)90 (457)039 6/26 (fimA type III)200 (593)117 (594)099 W83 (fimA type IV)148 (439)87 (441)096 HNA99 (fimA type V)191 (567)111 (563)094 ATCC29523 (Serotype a)61 (181)45 (228)019 Y4 (serotype b)100 (297)69 (350)020 AUNY67 (Serotype c)87 (258)67 (340)0044* ATCC2651192 (273)64 (325)025 ATCC3356355 (163)40 (203)024 ATCC2558650 (148)64 (325)Rabbit polyclonal to IPMK predictor of unfavorable outcome following stroke (OR?=?312, 95% CI =?155C629; ATCC33277 (fimA type I)073044C120022 ATCC29523 (serotype a)063031C129020 Y4 (serotype b)104055C199090 AUNY67 (serotype c)121062C236057 ATCC25586163087C303013 ATCC10953312155C6290002* ATCC35405116046C293075 ATCC33238145080C264022 ATCC23834098047C206097 Open in a separate window *Based on 20 factors included for analysis with has been identified as one of the main pathogens responsible for progression of periodontitis [26] and its fimbriae are considered to be an important virulence factor [27]. Furthermore, that bacterium has been.