Importantly, infection of mice lacking COX-1, but not COX-2 activity resulted in a defect in immunoglobulin class-switching and a lack of studies suggested that products of COX might be involved in the regulation of antibody production (12) and both COX isozymes have been implicated in antibody production (17, 18)

Importantly, infection of mice lacking COX-1, but not COX-2 activity resulted in a defect in immunoglobulin class-switching and a lack of studies suggested that products of COX might be involved in the regulation of antibody production (12) and both COX isozymes have been implicated in antibody production (17, 18). most commonly used drugs in the world (19, 20), a thorough understanding of their LED209 effects on immunity is lacking. To address these issues we utilized a murine model of Lyme disease, caused by infection with the spirochete, infection of both humans and animals is the most common vector-borne disease in both the U.S. and in Europe (21). When untreated with antibiotics near the time of infection, 60% of individuals develop a severe arthritis, the intense pain FGF21 and swelling of which is commonly treated with COX-specific inhibitors or traditional nonsteroidal anti-inflammatory drugs (tNSAIDs) (22). Here we show that murine B cells, in response to stimulation expressed both COX isozymes, and inhibition of either isozyme affected B cell eicosanoid production. studies utilizing COX-1 or -2-specific inhibitors or COX-specific knock-out mice demonstrated that COX-1 activity was required for the LED209 generation of a full antiIgG response. Further analysis demonstrated that COX-1 was necessary for the development of GC and LED209 the production of normal IL-6 and IL-17 levels in response to infection. Our results demonstrate a critical role for COX-1 in the regulation of GC formation and the generation of humoral immunity up-stream of IL-6 and IL-17 production during the response to infection. Additionally, these data suggest that commonly used NSAIDs may affect the ability of the hosts immune system to effectively protect against pathogens. Materials and Methods Animals Female C3H/HeJ (C3H) mice, 4C6 weeks of age, were purchased from The Jackson Laboratory (Bar Harbor, ME). COX-2 heterozygous mice (B6;129S7-spirochetes at a multiplicity of infection (MOI) =1, total antigen (BbAg, 5g/mL), arachidonic acid (10M, Cayman Chemical, Ann Arbor, MI), or were untreated. The concentration of antigen used has been shown to activate B cells and induce their proliferation and differentiation into plasma cells (23). B cells were stimulated with arachidonic acid (AA) as a positive control for COX-1 stimulation (24). For the analysis of FP and TP receptor expression, B cells were stimulated with an MOI = 1 and collected at the indicated time points. For FP antagonism the FP antagonist AL-8810 (Cayman Chemical) was dissolved in 100% ethanol as a stock solution and stored at ?20C until dilution to the working concentration of 50 M in cell culture medium. B cells were pre-incubated with vehicle or antagonist 30 minutes before the addition of stimulus and supernatants were harvested 7 days later. Cell viability was determined by trypan blue staining. Inhibition of cyclooxygenase-1 LED209 or -2 Celecoxib (LKT Laboratories, Inc, St. Paul, MN) and SC-560 (Cayman Chemical) were dissolved in 100% ethanol/0.01% Tween-20 or 100% ethanol alone, respectively, as stock solutions and stored at ?20C until dilution to the working concentration of 1 1 M in cell culture medium. Treatment of cells with COX inhibitor concentrations greater than 10M increased cell death in dose-response studies. B cells were pre-incubated with inhibitors or vehicle for 30 minutes before the addition of stimuli. For inhibition of COX-2, celecoxib was incorporated into a normal laboratory diet (Research Diets, New Brunswick, NJ) as described (25). Animals were fed celecoxib chow beginning day -1 of infection with and control animals were fed normal LED209 rodent chow (Purina PicoLab 5053, Purina Mills, St. Louis, MO). For COX-1 inhibition, dilutions of SC-560 were mixed daily in 200L sterile PBS and animals were treated once daily by oral gavage for a final dosage of 10 mg kg?1 day?1. RNA and RT-PCR Total RNA was extracted with TRIzol reagent (Invitrogen Corp, Carlsbad, CA) according to the manufacturers protocol. One-step RT-PCR was performed using the EZ RT-PCR kit (Applied Biosystems, Foster City, CA) and 100ng of total RNA with the ABI Prism 7700 Sequence Detection System (Applied Biosystems). The mouse gene, a single copy gene, was used as an endogenous control as described previously (26). COX-1 and -2 primer sequences were described previously (17). RT-PCR conditions were: 50C for 15 min, 60C for 30 min, 95C for 10 min, and 45 cycles of 95C for 30 sec and 60C for 1 min.. SDS-PAGE/Western analysis Total.