(D) Relative regularity of cells produced from AA&R and DT aorta of Apoe?/?mice in HCD and NCD composing the 12 clusters; Body S3: Temperature map of best differentially portrayed genes among all discovered 12 clusters; Body S4: Move terms enrichment evaluation of AA&R and DT aorta produced clusters of Apoe?/? on HCD and NCD. cells produced from AA&R of on NCD versus HCD. Dot size is certainly proportional to the real amount of genes overlapping with each Move term, as the altered mice on NCD versus HCD. Dot size is certainly proportional to the amount of genes overlapping with each Move term, as the altered 0.05. MKC9989 A linear regression evaluation was performed to evaluate the Alizarin reddish colored positive staining towards the percentage of LYVE1+ CCL24+ cells, or CCL24 levels, in the serum. 3. Results 3.1. The Site-Specific Development of Atherosclerosis Defines the Hypercholesteremia-Associated Transcriptional Signature The site-specific development of atherosclerotic lesions are observed in both murine models of atherosclerosis and in humans [40]. encoding CD11c, [42] and Slpi, shown to attenuate inflammatory cytokine production [43] (Figure 2F,G). The GO term enrichment analysis of cluster 0 showed the involvement of the identified cluster of monocytes/macrophages in the negative regulation of cell adhesion, wound healing, leukocyte chemotaxis, the negative regulation of cytokine production, and the regulation of cytokine secretion and blood coagulation (Figure S4A). Cluster 1, consisting of cells mainly derived from the AA&R, expressed a set of genes corresponding to a population of monocyte-derived dendritic cells (MoDC/DC) with a pro-inflammatory phenotype (Figure 2F,G) as shown by the high expression of Cxcl10, Ccr2, Hspa1b, Lilra5, and Ifit2 (Figure 2G and Figure S3). In line with the observed gene expression profile of cluster 1, the GO term enrichment analysis revealed signaling pathways linked to T-cell activation, the positive regulation of cell adhesion, cytokine TRKA production, cell-to-cell adhesion, and leukocyte migration, as well as antigen processing and presentation via MHC class II (Figure S4B). This indicates that cluster 1 is an important population involved in antigen presentation and T-cell activation in the atherosclerotic plaques [44]. Cluster 2 exhibited a gene expression profile of macrophages as shown by the expression of Ednrb, Fcna, Retnl, and Cd209f, as well as genes that are typical for resident macrophages (Lyve1 and FCGR1A (CD64)) [5] (Figure 2G and Figure S3). Cluster 3 emerges as a population of macrophages characterized by the expression of Pcp4l1, Serpine1, Nelfcd, Trim36, Prc1, and Lgals3 (Galectin-3, also known as Mac-2) (Figure 2G and Figure S3). The GO term enrichment analysis of cluster 3 showed a positive regulation of protein kinase activity and a negative regulation of cell migration, motility, and locomotion, as well as a positive regulation of MAP kinase activity and a negative regulation of blood coagulation and platelet activation, probably linked to an important role in coagulation (Figure S4C). Cluster 4 was present only in the DT aortas of and known as resident macrophage markers [53,54]. Interestingly, cluster 2 expressed which is typical for a subtype of alternatively activated macrophages MKC9989 called Mhem/M (Hb) [55] and were first described in areas with intraplaque hemorrhages [56]. Furthermore, CD163 positive macrophages have been found in vulnerable human carotid plaques, which support the notion that expression could contribute to clinical events [55]. Cluster 2 also expressed (Figure 2G), which is emerging as a new potential negative regulator of collagen production [57] and genes typically expressed in M2-like macrophages, such as and also known as [2], as well as expressed by alternatively activated macrophages [58], (sepp1) [59], encoding CXCL4, MKC9989 and the inflammatory gene [60] (Figure 3B). The GO term enrichment analysis of cluster 2 revealed an upregulation of gene sets involved in leukocyte and lymphocyte migration/chemotaxis and the regulation of the humoral immune response, the acute inflammatory response, and complement activation (Figure 3C). Open in a separate window Figure 3 Cluster 2 gene expression signature. Gene sets were overlaid on single cells on a tSNE plot to identify the cell identity of cluster 2 with an enrichment of indicated gene sets: MKC9989 (A) Resident-like Macrophages; and (B) top eight expressed genes of cluster 2. (C) Bubble plot GO term enrichment analysis of cells of cluster 2. Dot size is proportional to the number of genes overlapping with each GO term, while the adjusted = MKC9989 8 mice per group and *** 0.001. Representative dot plots show the gating strategy illustrating LYVE1+ res-like macrophage marker expression in.
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