For all those cSiO2-treated groups, Spearman values were calculated by correlating total Ab-score values with markers for ELS development assessed in lung tissues of mice collected 1, 5, 9, or 13 weeks PI. the microarray panel for vehicle (VEH)- or cSiO2-uncovered mice fed CON, low DHA, or high DHA diets. Total IgM Ab-score data ( Ab-score) calculated as the sum of AAbs belonging to a particular category (observe Table 1). One-way ANOVA was used to compare experimental groups at selected time points followed by Tukey HSD method as multiple comparison test. Data are mean SEM. Symbols indicate significant difference (p0.05) as follows: * for cSiO2/ PG 01 CON vs. VEH/ CON; # for cSiO2/ CON vs. cSiO2/ Low DHA; and for cSiO2/ CON vs cSiO2/ High DHA. Supplementary Physique 4. DHA supplementation dose-dependently suppresses cSiO2-induced IgM PG 01 lupus-associated AAbs in BALF (A) and plasma (B) of mice over time. Relative IgM AAb responses for selected lupus-associated AAgs were determined by dividing individual Ab-score by the mean Ab-score for VEH-treated mice fed control diet at week 1 PI. One-way ANOVA was used to compare experimental groups at selected time points followed by Tukey HSD method as multiple comparison test. Data are mean SEM. Symbols indicate significant difference (p0.05) as follows: * Cd86 for cSiO2/ CON vs. VEH/ CON; # for cSiO2/ CON vs. cSiO2/ Low DHA; and for cSiO2/ CON vs cSiO2/ High DHA. Supplementary Physique 5. DHA intake inhibits cSiO2-induced expression of IgM AAbs in BALF (A) and plasma (B) specific for AAgs associated with other autoimmune diseases. AAbs included those associated with rheumatoid arthritis (collagen II, fibrinogen IV, AAbs fibrinogen S, fibronectin, and vimentin), Sj?gren’s syndrome (-fodrin), systemic sclerosis (topoisomerase I)), vasculitis (MPO and PR3), myositis (Mi-2, TIF1-, MDA5), autoimmune hepatitis (LC-1), and celiac disease (TTG). Relative IgM AAb responses for selected AAgs were determined by dividing individual Ab-score by the mean Ab-score for VEH-treated mice fed control diet at week 1 PI. One-way ANOVA was used to compare experimental groups at selected time points followed PG 01 by Tukey HSD method as multiple comparison test. Data are mean SEM. Symbols indicate significant difference (p0.05) as follows: * for cSiO2/ CON vs. VEH/ CON; # for cSiO2/ CON vs. cSiO2/ Low DHA; and for cSiO2/ CON vs cSiO2/ High DHA. Supplementary Physique 6. IgG and IgM AAb responses in BALF positively correlate with cSiO2-induced gene expression in lung. For all those cSiO2-treated groups, Spearman values were calculated by correlating total Ab-score values ( Ab-Score) in PG 01 BALF (A) and plasma (B) with Z scores of gene expression in selected immune pathways at 1, 5, 9, or 13 weeks. Total IgG Ab-score ( Ab-Score) data calculated as the sum of AAbs belonging to a particular category (observe Table 1). Significant correlation values (p0.05) are represented as circles colored by the correlation value (blue, positive; reddish, negative); PG 01 non-significant correlations are indicated by blank cells. Supplementary Physique 7. IgM AAb responses in BALF (A) and plasma (B) positively correlate with markers of ELS development. For all those cSiO2-treated groups, Spearman values were calculated by correlating total Ab-score values with markers for ELS development assessed in lung tissues of mice collected 1, 5, 9, or 13 weeks PI. Total IgM Ab-score ( Ab- Score) data calculated as the sum of AAbs belonging to a particular category (observe Table 1). Markers for ELS development measured as the percentage of positively stained tissues (CD3, CD45R, and CD21/35) and scores of phenotypic endpoints (histopathology scores, lymphoid aggregates, ectopic lymphoid tissue). Significant correlation values (p0.05) are represented as circles colored by the correlation value (blue, positive; reddish, negative); non-significant correlations are indicated by blank cells. Supplementary Physique 8. AAb responses correlate positively with tubular proteinosis and CD45R positivity (B cells) in the kidney. (A) For all those cSiO2-treated groups, Spearman values were calculated by correlating Ab-Score values with histopathology score of tubular proteinosis in kidney of corresponding animals at 13 weeks PI. Significant correlation values (p0.05) are represented as circles colored by the correlation value (blue, positive; reddish, negative); non-significant correlations are indicated by blank cells..
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