4 The effects of the injection of 2AP on spatial memory space

4 The effects of the injection of 2AP on spatial memory space. LSD post-hoc test or Students non-significant Reduction of hippocampal neurogenesis and remote spatial memory space by extra 2AP We next investigated the effect of 2AP injection on adult hippocampal neurogenesis and the spatial memory space process. The number of BrdU-positive and Ki67-bad cells in the DG was significantly decreased from the injection of 2AP in comparison to the control mice (Fig.?3a, b). Accordingly, the number of Dcx-positive cells was decreased by extra 2AP (Fig.?3c, d). 2AP was injected into the brain after the 1st day time of training in the MWM test. Probe checks Senexin A were performed on the second day time of teaching (30?min after teaching), and then at 1?month (Fig.?4a). In the training for the MWM test, there was little difference in the latency to the platform between the 2AP-injected mice and the control mice (Fig.?4b). In the probe test at 30?min after teaching, the time in the prospective quadrant was longer than that in the other quadrants in both groups of mice, and the time in each quadrant did not differ between them, indicating that both mice remembered where the platform had been (Fig.?4c). The swimming velocity of the 2AP-injected mice and control mice did not differ to a statistically significant degree (16.5??0.5 and 16.4??0.7?cm/s, respectively). In the probe test at 1?month after the training sessions, Senexin A there was no difference in the time the 2AP-injected mice spent in each quadrant, while the time the control mice spent in the prospective quadrant was markedly longer than that in the opposite quadrant (Fig.?4d). These results suggest that extra 2AP suppresses hippocampal neurogenesis, as well as spatial memory space retention and recall. Open in a separate windows Fig. 3 The effects of the injection of 2AP on adult hippocampal neurogenesis. a Representative images of immunostaining of BrdU and Ki67. 2AP or saline was intracerebroventricularly injected in 12-week-old C57BL/6J mice, 2?h after the first intraperitoneal injection of BrdU, and BrdU was administered at 24-h intervals for 7?days. Coronal brain slices were immunostained with antibodies. Arrow mind show Ki67+ cells. Level pub: 50?m. b The numbers of BrdU+/Ki67? cells in the DG were counted inside a blinded manner. c Representative images of immunostaining of Dcx. Level pub: 200?m. d The numbers of Dcx+ cells in the DG were counted inside a blinded manner. The ideals represent the mean??S.E. (saline: n?=?5, 2AP: n?=?6). Statistical significance Rabbit Polyclonal to ROCK2 was evaluated using College students t-test. *P?Senexin A 2AP in the cerebrospinal fluid in aged mice tended to become higher than that Senexin A in young mice (young mice: 1.0??0.4, old mice: 1.5??0.2; n?=?4/group), while the 2AP level in the plasma in old mice.