Author: lacbiosafety

After 1C2 weeks cells were stained for surface expression of CD4+ and CD8+ by flow cytometry and found to consist mainly (a lot more than 80%) of CD8+ T cells, and tested for antigen specificity by co-culturing with HLA-A11 expressing BLCLs in the current presence of NS3 peptides from DV1, 2, 3/4, or DMSO as control

After 1C2 weeks cells were stained for surface expression of CD4+ and CD8+ by flow cytometry and found to consist mainly (a lot more than […]

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Read counts 10 in all the samples were first removed and the remaining data were regularized log (rlog) transformed Statistical significance was calculated using default guidelines, and genes were selected based on log2 collapse change higher/less than 1

Read counts 10 in all the samples were first removed and the remaining data were regularized log (rlog) transformed Statistical significance was calculated using default […]

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Do the responsive -cell clusters remain robustly responsive in their gene expression patterns with more prolonged feeding? Do the less responsive clusters become more responsive with time? Are specific clusters more susceptible to apoptosis or dedifferentiation over time? Does the islet isolation process influence mRNA expression? In future studies, applying our annotation and clustering analyses should allow us to address these questions using timed feeding cohorts

Do the responsive -cell clusters remain robustly responsive in their gene expression patterns with more prolonged feeding? Do the less responsive clusters become more responsive […]

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